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MUSC
Dept. of Biochemistry
Crystallography Facility
Function
Facilities
Crystallization Lab.
X-ray Lab.
Molecular Graphics Lab.
Structures
Publications
MUSC access only
(MNA account)
Software Help
Under Construction
Schedule
Status
Use
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| This laboratory is where the data is collected. The
crystals, once cryo-protected, are then flash-cooled and mounted in the
cryo-stream on the Rigaku RU-H3 rotating anode X-ray generator (Figs 1, and 2). The cryo-stream is generated by
the X-stream cryo-stat (Fig1) and
maintains a temperature of 100 K around the crystal. Once the shutter is
opened the X-rays are focused by the Osmic Confocal optics (Fig.2 )and then collimated onto the
mounted crystal. The diffracted X-rays are then collected by the
RAXIS-IV++ image-plate detector (Figs 1
and 2). The images are then sent to
the data collection PC to be processed into a data set (Fig 3). |
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| Figure 1: Right hand side of the X-ray lab. The X-ray
generator is in the center and the water chiller to the right. |
Figure 2: A close-up of
data collection equipment.
Note the mounted crystal in the monitor (left hand corner)
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Figure 3: The PC that controls the data collection and
processes the images. An image is on the screen.
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